Part:BBa_K3286204

Two component system RpfCch + RpfG

The function of this composite part is that's a Two component system able to sense a diffused signalling factor (DSF)[1]. The sensor is called RpfC(ch) (BBa_K3286200).When DSF is present, the RpfCch will autophosphorylate and transfer the phosphate group to the enzyme RpfG. RpfG (BBa_K3286201) is a phosphodiesterase which is able to cleave c-di-GMP. This results in activation of this enzyme, which will decrease the amount of c-di-GMP in the cell.

1. H. Slater, A. Alvarez-Morales, C. E. Barber, M. J. Daniels, and J. Maxwell Dow, “A two-component system involving an HD-GYP domain protein links cell-cell signalling to pathogenicity gene expression in Xanthomonas campestris,” Mol. Microbiol., vol. 38, no. 5, pp. 986–1003, 2000.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1922
  Illegal PstI site found at 887
  Illegal PstI site found at 1493
  Illegal PstI site found at 2120
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1922
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 887
  Illegal PstI site found at 1493
  Illegal PstI site found at 2120
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1922
  Illegal BglII site found at 288
  Illegal BglII site found at 3362
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1922
  Illegal PstI site found at 887
  Illegal PstI site found at 1493
  Illegal PstI site found at 2120
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1922
  Illegal PstI site found at 887
  Illegal PstI site found at 1493
  Illegal PstI site found at 2120
  Illegal NgoMIV site found at 818
  Illegal NgoMIV site found at 909
  Illegal NgoMIV site found at 1736
  Illegal NgoMIV site found at 1831
  Illegal NgoMIV site found at 2890
  
• 1000
  COMPATIBLE WITH RFC[1000]